HRP Streptavidin 405210 from BioLegend

Product Specs
ItemHRP Streptavidin
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number405210
Quantity1 mL
ApplicationsELISA, ELISPOT, Immunohistochemistry (IHC), Western Blot (WB)
FormatBuffered solution containing bovine protein and preservatives (methylisothiazolone, bromonitrodioxane, and other active isothiazolones)
Conjugate/TagHRP
SpeciesHuman, Mouse, Rat, All Species
TypeStreptavidin
TargetBiotin

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) N-linked glycosylation is essential for anti-tumor activities of KIAA1324 in gastric cancerCell Death & DiseaseAugust 23, 2023Rebecca Yun, Eunji Hong, Junil Kim, Bora Park, Staci Jakyong Kim, Bona Lee, Yong Sang Song, Seong-Jin Kim, Sujin Park, Jin Muk KangUSA, B-1395-1) overnight at 4 °C and High Sensitivity Streptavidin-HRP (Biolegend, California, USA, 405210) for 1 h at room temperature. Then, they were detected using chemiluminescence (GE Healthcare Life Sciences,

(2) Insertion of an immunodominant T helper cell epitope within the Group A Streptococcus M protein promotes an IFN-γ-dependent shift from a non-protective to a protective immune responseFrontiers in ImmunologyAugust 15, 2023Shiva Emami, Thiago Rojas Converso, Jenny J. Persson, Bengt Johansson-Lindbom(BioLegend, RMG2b-1), and IgG2c (Southern Biotech, CAT 1079-08) and streptavidin-HPR (BioLegend, CAT 405210). BSA-coated wells served as negative controls and presented values represent the average of duplicate

(3) A Food Matrix Triggers a Similar Allergic Immune Response in BALB/c Mice Sensitized with Native, Denatured, and Digested OvalbuminLife (Basel)August 12, 2023Jesús Gilberto Arámburo-Gálvez, Raúl Tinoco-Narez-Gil, Aldo Alejandro Arvizu-Flores, Oscar Gerardo Figueroa-Salcido, José Antonio Mora-Melgem, Alma Rosa Islas-Rubio, Lilian Karem Flores-Mendoza, Veronica Lopez-Teros, Humberto Astiazaran-Garcia, Feliznando Isidro Cárdenas-Torres, et al.96-wells plates (Costar Assay plate, EE. UU), streptavidin-horseradish peroxidase (BioLegend. Cat. 405210), and tetramethyl benzidine TMB (Thermo Scientific, Cat. 34028) were used in the present study. 2.2.

(4) Tannins amount determines whether tannase-containing bacteria are probiotic or pathogenic in IBDLife science allianceMay 1, 2023He Q, Guo K, Wang L, Xie F, Zhao Q, Jiang X, He Z, Wang P, Li S, Huang Y, et al.incubated in a humidified chamber at room temperature for 30 min. After that, diluted Sav-HRP conjugates (405210; Biolegend) were applied appropriately to slides and incubated in a humidified chamber at room temperature.

(5) LMAN1 is a receptor for house dust mite allergensCell reportsMarch 28, 2023Miller MH, Swaby LG, Vailoces VS, LaFratta M, Zhang Y, Zhu X, Hitchcock DJ, Jewett TJ, Zhang B, Tigno-Aranjuez JT.overnight. After washing, plates were incubated with Streptavidin-HRP (Biolegend; San Diego, CA) (Cat# 405210) and developed using TMB substrate (Invitrogen; Waltham, MA) (Cat# 00420156). For testing blocking ability

(6) Design and preclinical evaluation of a universal SARS-CoV-2 mRNA vaccineFrontiers in ImmunologyMarch 23, 2023Jane Qin, Ju Hyeong Jeon, Jiangsheng Xu, Laura Katherine Langston, Ramesh Marasini, Stephanie Mou, Brian Montoya, Carolina R. Melo-Silva, Hyo Jin Jeon, Tianyi Zhu, et al.antibodies, Biotin-IFN𝝲 (Biolegend, Cat# 505714, clone R46A2) and Streptavidin-HRP (Biolegend, Cat# 405210) at RT for 1-2 hours, respectively. The plates were developed with 50 µL/well AEC development solution

(7) GPI-anchored ligand-BioID2-tagging system identifies Galectin-1 mediating Zika virus entryiScienceDecember 22, 2022Gao SS, Shi R, Sun J, Tang Y, Zheng Z, Li JF, Li H, Zhang J, Leng Q, Xu J, et al.from Southern Biotech (Birmingham, AL, USA). Mouse IgG1 (400165) isotype control and HRP-streptavidin (405210) were purchased from Biolegend (CA, USA). Alexa Fluor 647nm conjugated streptavidin (S-21374), Alexa

(8) Analysis tools for single-monomer measurements of self-assembly processesScientific ReportsDecember 1, 2022Maria Hoyer, Alvaro H. Crevenna, Radoslaw Kitel, Kherim Willems, Miroslawa Czub, Grzegorz Dubin, Pol Van Dorpe, Tad A. Holak, Don C. Lambprogress of the reaction was monitored with immunoblotting using HRP-conjugated streptavidin (Cat. #405210; BioLegend). After completion of the desthiobiotynilation reaction, both proteins were purified by size-exclusion chromatography

(9) Electrochemiluminescence in paired signal electrode (ECLipse) enables modular and scalable biosensingScience AdvancesSeptember 23, 2022Young Kwan Cho et al.antibodies, we added horseradish peroxidase–conjugated streptavidin (1:20,000 diluted in 0.1% BSA; #405210, BioLegend) to each well (50 μl), waited for 20 min (20°C), and washed the plate three times. Last,

(10) GLS1 is a Protective Factor in Patients with Ovarian Clear Cell Carcinoma and its Expression Does Not Correlate with ARID1A-mutated TumorsCancer Research CommunicationsSeptember 9, 2022Valentino Clemente et al.temperature followed by incubation with horseradish peroxidase streptavidin at a dilution of 1:125 (405210, BioLegend) for 30 minutes at room temperature. After the staining was developed with 3,3′-diaminobenzidine

(11) MicroID2: A Novel Biotin Ligase Enables Rapid Proximity-Dependent ProteomicsMolecular & cellular proteomics : MCPJuly 1, 2022Johnson BS, Chafin L, Farkas D, Adair J, Elhance A, Farkas L, Bednash JS, Londino JD.rapamycin human FRB domain; ENZO), FLAG (ANTI FLAG M2; Fisher), and streptavidin–HRP (catalog no.: 405210; BioLegend). Immunofluorescence Immunofluorescence was performed as described previously (14). Briefly,

(12) Two-Component Spike Nanoparticle Vaccine Protects Macaques from SARS-CoV-2 InfectionSSRN Electronic JournalMarch 30, 2022Philip J. M. Brouwer et al.Thermo Fisher Cat# 10010023 PEI MAX Polysciences Cat# 24765-1 HRP-labeled streptavidin Biolegend Cat# 405210 3,30 ,5,50 -tetranethylbenzidine Sigma-Aldrich Cat# T4444 Squalene Emulsion adjuvant Polymun Scientific N/A Polyinosinic-polycytidylic

(13) A Spike Protein-Based Subunit SARS-CoV-2 Vaccine for Pets: Safety, Immunogenicity, and Protective Efficacy in Juvenile CatsFrontiers in Veterinary ScienceMarch 14, 2022Kairat Tabynov, Madiana Orynbassar, Leila Yelchibayeva, Nurkeldi Turebekov, Toktassyn Yerubayev, Nurali Matikhan, Tlektes Yespolov, Nikolai Petrovsky, Kaissar Tabynovtemperature with shaking). After additional washing (×4), plates were incubated with HRP streptavidin (#405210, BioLegend, 1:1000, 100 μl per well) for 30 min at room temperature with shaking. Finally, plates were

(14) An adjuvanted subunit SARS-CoV-2 spike protein vaccine provides protection against Covid-19 infection and transmissionNPJ vaccinesFebruary 23, 2022Tabynov K, Turebekov N, Babayeva M, Fomin G, Yerubayev T, Yespolov T, Li L, Renukaradhya GJ, Petrovsky N, Tabynov K.temperature with shaking). After additional washing (4x), plates were incubated with HRP Streptavidin (# 405210, BioLegend, 1:1000, 100 µl/well) for 30 min at room temperature with shaking. Finally, plates were washed

(15) Characterization and modulation of surface charges to enhance extracellular vesicle isolation in plasmaTheranosticsJanuary 31, 2022Hyun-Kyung Woo, Young Kwan Cho, Chang Yeol Lee, Haeun Lee, Cesar M. Castro, Hakho Leeincubated for 1 h at RT. After washing twice, HRP-conjugated streptavidin (1:20,000 diluted in 0.1% BSA; #405210, BioLegend) were loaded (50 μL/well) and incubated for 20 min at RT. After washing three times, 100

(16) Hydroxylation of N-acetylneuraminic Acid Influences the in vivo Tropism of N-linked Sialic Acid-Binding Adeno-Associated Viruses AAV1, AAV5, and AAV6Frontiers in MedicineDecember 21, 2021Estrella Lopez-Gordo, Alejandro Orlowski, Arthur Wang, Alan Weinberg, Susmita Sahoo, Thomas Weber60 min at r.t. Next, the membrane was washed as before, incubated with HRP-streptavidin (cat. no.: 405210; BioLegend) in a 1:2,000 dilution for 60 min at r.t., washed again, and developed using the SuperSignal

(17) Local DNA shape is a general principle of transcription factor binding specificity in Arabidopsis thalianaNature CommunicationsDecember 1, 2021Janik Sielemann, Donat Wulf, Romy Schmidt, Andrea Bräutigamwith 1:5000 solution of an anti-biotin HRP-conjugated antibody (BioLegend, San Diego, CA, USA; Cat.: 405210; Batch: B293545; 1:5000) in TBST with 5% BSA. Detection was performed using PierceTM ECL Western Blotting

(18) AF10 (MLLT10) prevents somatic cell reprogramming through regulation of DOT1L-mediated H3K79 methylationEpigenetics & ChromatinDecember 1, 2021Deniz Uğurlu-Çimen et al.3% FBS and 0.3% Triton X-100. Followed by incubation with streptavidin-HRP (Biolegend, catalog no. 405210, 1:500). Staining was developed with the DAB peroxidase substrate solution (0.05% 3,3′-diaminobenzidine

(19) Biochemical Characterisation of Human Transglutaminase 4International Journal of Molecular SciencesNovember 18, 2021Zsuzsa Csobán-Szabó, Bálint Bécsi, Saïd El Alaoui, László Fésüs, Ilma Rita Korponay-Szabó, Róbert Királyand 0.43 M β-mercaptoethanol). Incorporated BPA was analysed by Western blot using Streptavidin/HRP (405210, BioLegend San Diego, CA, USA, 1/2500). The incorporation reaction was repeated three times to identify

(20) Lymph node-resident dendritic cells drive T(H)2 cell development involving MARCH1Science immunologyOctober 15, 2021Castellanos CA, Ren X, Gonzalez SL, Li HK, Schroeder AW, Liang HE, Laidlaw BJ, Hu D, Mak ACY, Eng C, et al.washed with PBST and incubated with horseradish peroxidase (HRP) streptavidin (BioLegend, catalog no. 405210) for 30 min at RT. Plates were washed with PBST and developed with trimethylboron substrate (eBioscience,

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5/20/2020Best HRP to Detect Biotinylated Antibodies Abhijit NirwaneStreptavidin binds to biotin with high affinity. Streptavidin-HRP is useful for detecting biotinylated antibodies, e.g., in ELISA, IHC or Western blotting.

HRP Streptavidin from BioLegend

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HRP Streptavidin from BioLegend

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